Development and application of triple antibody sandwich enzyme-linked immunosorbent assays for begomovirus detection using monoclonal antibodies against Tomato yellow leaf curl Thailand virus

Table 4 Percentage identity between CP amino acid sequences of begomoviruses with different immunoreactivity to MAbs M1 and D2

Begomovirus	Immuno-reactivity TAS-ELISA		Sequence identity between CP amino acid (%)
	Immuno-reactivity TAS-ELISA		TYLCTHV-[To-SB-2]	TbLCYnV-[To-CM]	PepLCV-[Pe-SP-11]	AYVV-[To-SB-9]	SLCCNV-[Pk-NP]	ToLCNDV-[Cu-KK]	BYVMV-[Ok-CM]	PepYLCIV-[Pe-PN-1]	TYLCKaV-[Ep-CM]	TYLCCNV-[BS-1]*	TYLCIDV-[Lem-05]*	TYLCV-[IL-Rep-86]*
	M1	D2	TYLCTHV-[To-SB-2]	TbLCYnV-[To-CM]	PepLCV-[Pe-SP-11]	AYVV-[To-SB-9]	SLCCNV-[Pk-NP]	ToLCNDV-[Cu-KK]	BYVMV-[Ok-CM]	PepYLCIV-[Pe-PN-1]	TYLCKaV-[Ep-CM]	TYLCCNV-[BS-1]*	TYLCIDV-[Lem-05]*	TYLCV-[IL-Rep-86]*
TYLCTHV-[To-SB-2]	+	+		93	81	81	79	78	78	72	72	81	75	73
TbLCYnV-[To-CM]	+	+			81	83	81	81	80	73	73	80	74	74
PepLCV-[Pe-SP-11]	±	±				89	82	81	83	75	75	82	78	78
AYVV-[To-SB-9]	±	±					84	85	84	76	76	83	79	79
SLCCNV-[Pk-NP]	-	+						96	91	73	72	77	77	76
ToLCNDV-[Cu-KK]	-	+							91	74	73	77	78	76
BYVMV-[Ok-CM]	-	-								74	75	78	77	75
PepYLCIV-[Pe-PN-1]	-	-									92	73	72	71
TYLCKaV-[Ep-CM]	-	-										73	71	69
TYLCCNV-[BS-1]*	nd	nd											76	77
TYLCIDV-[Lem-05]*	nd	nd												76
TYLCV-[IL-Rep-86]*	nd	nd

Asterisks indicate the related tomato yellow leaf curl begomovirus species for phylogenetic comparison. nd not determined

ISSN: 1743-422X