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Table 2 Detection and identification of begomoviruses in field samples by TAS-ELISA, PCR and DNA sequence analysis

From: Development and application of triple antibody sandwich enzyme-linked immunosorbent assays for begomovirus detection using monoclonal antibodies against Tomato yellow leaf curl Thailand virus

Host No. of samples tested No. of samples positive by PCR TAS-ELISAe Species based on sequence analysis of cp gene
No. of samples positive by M1 No. of samples positive by D2 TYLCTHV TbLCYnV ToLCNDV SLCCNV PepLCV AYVV BYVMV PepYLCIV TYLCKaV
Tomato 37 27 15 21 11a 4a 1b    9(5c,4d)    2d
Cucumber 39 29 0 29    29b       
Loofah 6 1 0 1    1b       
Ivy gourd 1 1 0 1    1b       
Melon 9 4 0 4    2b 2b      
Pumpkin 5 5 0 5     5b      
Wax gourd 2 2 0 2     2b      
Eggplant 5 5 0 0          5d
Okra 5 5 0 0        5d   
Pepper 34 34 0 9      24(9c,15d)    8d 2d
Weed 4 4 1 1   1a    1d 2d    
Total 147 117 15 73 11 5 34 9 25 11 5 8 9
  1. aPositive with M1 and D2
  2. bPositive with D2
  3. cWeakly positive with M1 and/or D2
  4. dNegative with M1 and D2
  5. eSample positive by MAb M1 was also positive by MAb D2