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Fig. 1 | Virology Journal

Fig. 1

From: Development and application of triple antibody sandwich enzyme-linked immunosorbent assays for begomovirus detection using monoclonal antibodies against Tomato yellow leaf curl Thailand virus

Fig. 1

Analyses of the recombinant TYLCTHV-CP protein by SDS-PAGE and western blotting. a SDS-PAGE analysis of the recombinant TYLCTHV-CP protein. Lysate of non-induced E. coli cells harboring pQE30-TYLCTHV-CP (Lane 1); lysate of IPTG-induced E. coli cells harboring pQE30-TYLCTHV-CP (Lane 2); and the purified recombinant TYLCTHV-CP (Lane 3) were analyzed by 12% SDS-PAGE and subsequent Coomassie blue staining. The Low-Range SDS-PAGE Standard (Bio-Rad, Richmond, CA, USA) (Lane M) was used as the molecular weight marker. b Western blot analysis of the recombinant TYLCTHV-CP. The purified recombinant TYLCTHV-CP protein was analyzed by western blotting using a rabbit polyclonal antibody to begomovirus (Lane 1). The RainbowTM protein molecular weight marker was included (Lane M)

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