Fig. 6
From: Pathogenesis of virulent and attenuated foot-and-mouth disease virus in cattle
FMDV generates microvesicles within the palatine tonsil of cattle during the clinical phase of disease. a Low magnification image demonstrates the architecture of a large tonsillar crypt delineated by cytokeratin-positive (green) squamous epithelium. Dashed box indicates region of interest (ROI) within crypt wall containing a focus of FMDV (red)-infected cells forming a microvesicle. ROI is featured at higher magnification in 6b-d including different detection channels in each panel. b Inclusion of FMDV VP1 (red) and cytokeratin (green) channels demonstrates disruption of epithelial architecture with cavitation/vesiculation. Many of the FMDV-containing cells are also cytokeratin-positive (epithelial cells). c Inclusion of FMDV VP1 (red), CD11c (turquoise), and MHC II (purple) demonstrates that some of the FMDV-containing cells are also individually- or double-positive for these markers of monocytoid, phagocytic, and antigen presenting cells. d Simultaneous viewing of all 4 channels demonstrates that within the vesicular cavity, FMDV-containing cells of distinct phenotypes are interspersed and in close proximity. Multichannel immunofluorescence microcopy. (animal ID 938, tissue ID palatine tonsil) (magnification: a 4×, b-d 40×)