Fig. 5
From: Pathogenesis of virulent and attenuated foot-and-mouth disease virus in cattle
Distribution and morphology of pulmonary infection was similar for FMDV-WT at 48hpa and FMDV-Mut at 72hpa. Microscopic distribution of FMDV structural protein VP1 (red), cytokeratin (green), and CD11c (turquoise) in lungs of cattle infected with FMDV-WT (a-d) or FMDV-Mut (e-h). a & e Between 48 and 72hpa both viruses generated poorly demarcated foci of infected cells, and similarly formed vesicle-like cavitations comprised of acantholytic cells and debris. Foci of infection were more abundant in animals infected with FMDV-WT. b-d and f-h Higher magnification and selective channel combinations demonstrate similar co-localization patterns for both viruses. FMDV VP1 (red) predominantly localizes within cytokeratin-positive pulmonary epithelial cells (green), but not with CD11c monocytoid cells (turquoise). Acantholytic cells are cytokeratin-positive. Few infected cells contain neither cytokeratin nor CD11c (phenotype undetermined). Multichannel immunofluorescence microcopy. (FMDV-WT animal ID 960, tissue ID Lung; FMDV-Mut animal ID 1027, tissue ID Lung) (magnification: a & e 10×, b-d & f-h 40×)