Fig. 4
From: Pathogenesis of virulent and attenuated foot-and-mouth disease virus in cattle
At 24 hpa, distribution and morphology of primary infection is similar for FMDV-WT and FMDV-Mut. Microscopic distribution of FMDV structural protein VP1 (red) cytokeratin (green), and CD11c (turquoise) in nasopharyngeal mucosa of cattle infected with FMDV-WT (a-d) or FMDV-Mut (e-h). a & e Both viruses similarly infected superficial cytokeratin-positive nasopharyngeal mucosal epithelial cells. Dashed-lined boxes indicate regions of interest (ROI) shown at higher magnification in b-d, f-h. b-d and f-h Higher magnification and selective channel combinations demonstrate similar co-localization patterns for both viruses. FMDV VP1 (red) predominantly localizes within cytokeratin-positive epithelial cells (green), but not with CD11c (turquoise). Few infected cells contain neither cytokeratin nor CD11c (phenotype undetermined). Multichannel immunofluorescence microcopy, (FMDV-WT animal ID 930, tissue ID Dorsal nasopharynx –Rostral; FMDV-Mut animal ID 699, tissue ID Dorsal soft palate -Caudal) (magnification: a & e 20×, b-d & f-h 40×)