Fig. 2
From: Pathogenesis of virulent and attenuated foot-and-mouth disease virus in cattle
Detection of FMDV-WT by immunomicroscopy in bovine nasopharynx at 6 h post aerosol-inoculation. a Low magnification view demonstrates tissue architecture of the caudal aspect of the dorsal soft palate including surface epithelium (Ep), epithelial crypts (Cr), lymphoid follicles (LF) of mucosa-associated lymphoid tissue, and secretory glands (Gl). Two regions of interest (ROI) are centered on an epithelial crypt with a prominent subepithelial lymphoid follicle: solid-lined box indicates ROI highlighted in 1b; dashed box indicates ROI highlighted in 1c. Immunohistochemistry (IHC) with anti-FMDV capsid monoclonal antibody, micropolymer alkaline phosphatase detection system with hematoxylin counterstain. b Earliest microscopic detection of FMDV consisting of few cells containing FMDV VP1 within a shallow depression within the wall of an epithelial crypt. IHC with anti-FMDV capsid monoclonal antibody, micropolymer alkaline phosphatase detection system. c Serial section of tissue shown in a-b demonstrating few cells containing FMDV VP1 (red) within epithelium (green) or adhered to epithelial surface of crypt. Multichannel immunofluorescence microcopy, (animal ID 1002, tissue ID Dorsal soft palate -Caudal) (magnification: a 2×, b 40×, c 10×)