- Each column and the numbers in first four rows represents a single specific nucleotide position in the LCR. The fifth row shows the nucleotide (indicated by the standard letter of its nitrogenous bases; (A) adenine, (C) cytosine, (G) guanine, and (T) thymine) at each specific position for the LCR of the prototype HPV16 isolates, NC001526. The sixth row is the sequence of the SiHa positive control. Alphanumeric in column are NCBI BLAST obtained isolates of known lineage. The following were the indication:
variation unique to isolates of this study, 
variations that differentiate known HPV16 lineages, 
same nucleotide as prototype isolate, 
deletion of nucleotide at that position. Isolates’ GenBank accession numbers are shown in Additional file 1: Table S1
variation unique to isolates of this study, 
variations that differentiate known HPV16 lineages, 
same nucleotide as prototype isolate, 
deletion of nucleotide at that position. Isolates’ GenBank accession numbers are shown in Additional file