Fig. 4From: Specific and quantitative detection of Human polyomaviruses BKPyV and JCPyV in the healthy Pakistani populationReal time assay for the quantification of JCPyV in healthy blood samples. The qPCR was performed by using 30 ng DNA of each sample and virus copy number was determined by comparing with standard curve of known JCPyV dilutions. a Amplification plots of some representative positive samples for JCPyV. b The amplified product of these representative positive samples was analyzed by gel electrophoresis. The amplified product showed band of 177 bps corresponding to JCV LT. Lane 1: molecular mass marker; Lane 2–14: representative positive samples for JCPyV; Lane 15: negative controlBack to article page