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Fig. 3 | Virology Journal

Fig. 3

From: Specific and quantitative detection of Human polyomaviruses BKPyV and JCPyV in the healthy Pakistani population

Fig. 3

Real time assay for the quantification of BKPyV in healthy blood samples. The qPCR was performed by using 30 ng DNA of each sample and virus copy number was determined by comparing with standard curve of known BKPyV dilutions. a Amplification plots of some representative positive samples for BKPyV. b The amplified product of these representative positive samples was analyzed by gel electrophoresis. The amplified product showed band of 151 bps corresponding to BKPyV LT. Lane 1: molecular mass marker; Lane 2–13: representative positive samples for BKPyV; Lane 14: negative control

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Virology Journal

ISSN: 1743-422X