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Fig. 3 | Virology Journal

Fig. 3

From: Preliminary study of the UL55 gene based on infectious Chinese virulent duck enteritis virus bacterial artificial chromosome clone

Fig. 3

Comparative characterization of DEV CHv-BAC-G and its wild type virus. a-c Growth curves of wild type DEV CHv and reconstitute virus DEV CHv-BAC-G. DEF cells were infected at an MOI of 0.02, the TCID50 titer of infected supernatant, cells and mixture of cells cultures were titrated at the indicated time points. All titrations were carried out in three independent experiments. d Plaque area measurement of DEV CHv and DEV CHv-BAC-G. Means and standard deviations of plaques diameter of each strain were measured with ImageJ software. The Mean of plaque areas of DEV CHv was set at 100%. Standard deviations are shown with the error bar. NS: no significant difference ((t-test, p > 0.05). e, f Transmission electron microscopic examination of purified wild type and reconstitute DEV virions. Red box: The virion structure of each virus was scaled up for observation

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