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Fig. 3 | Virology Journal

Fig. 3

From: Glucose-regulated protein 78 demonstrates antiviral effects but is more suitable for hepatocellular carcinoma prevention in hepatitis B

Fig. 3

GRP78 promoted the cytosolic retro-transportation of P22 in HepG2.2.15 cells. HepG2.2.15 cells were regularly cultivated and transfected with pGRP78 (GRP78-expressing recombinant plasmid generated from pCMV6-XL5) or siRNA targeted GRP78 (siGRP78) or their controls, pCMV6-XL5 (pVector) and siRNA control (siControl). a GRP78 significantly inhibited HBeAg secretion. Results are expressed as n-fold times the supernatant HBeAg level of test groups over those of controls. * P < 0.05, **P < 0.01. b GRP78 promoted the retro-transportation of HBeAg precursor (P22) from ER to cytosol. Total cytosolic proteins (cytosol) and non-cytosolic proteins (non-cytosol) were separately extracted. The rest bands that varied as P22 or HBeAg are core-related peptides with modification or degradation in different degree

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Virology Journal

ISSN: 1743-422X