Fig. 2

GRP78 did not make use of the IFN-β1-RNase L pathway in HepG2.2.15 cells. HepG2.2.15 cells were regularly cultivated and transfected with pGRP78 (GRP78-expressing recombinant plasmid generated from pCMV6-XL5) or siRNA targeted GRP78 (siGRP78) or their controls, pCMV6-XL5 (pVector) and siRNA control (siControl). a GRP78 regulation did not significantly affect the mRNA levels of IFN-β1 and RNase L genes. Results are expressed as n-fold times the IFN-β1 or RNase L level of test groups over those of controls. b GRP78 regulation did not significantly affect the protein level of IFN-β1. c The mRNA levels of IFN-β1 and RNase L genes in HepG2.2.15 cells were much lower than those in HepG2 cells (parent cells of HepG2.2.15 cells). Results are expressed as n-fold times the IFN-β1 or RNase L level of test groups over those of controls. **P < 0.01. d The protein level of IFN-β1 in HepG2.2.15 cells was much lower than that in HepG2 cells