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Table 7 AIV, NDV, IBV and IBDV detection in clinical specimens with use of different tests

From: New oligonucleotide microarray for rapid diagnosis of avian viral diseases

Result Real-time RT-PCR Microarray Virus isolation in chicken embryos
Positive 117 (95.90%) 118 (96.72%) 118 (96.72%)
Falsepositive 0 0 0
Falsenegative 1 (0.82%) 1 (0.82%) 1 (0.82%)
Negative 4 (3.29%) 3 (2.46%) 3 (2.46%)
Total 122 (100%) 122 (100%) 122 (100%)
SN, at 95% CI 99.15% (97.30–100%) 99.16% (97.36–100%) Standard method for comparison
SP, at 95% CI 100% 100%
PPV, at 95% CI 100% 100%
NPV, at 95% CI 80.00% (71.96–88.04%) 75.00% (66.29–83.71%)
  1. Abbreviations: SN Sensitivity, SP Specificity, PPV Positive Predictive Value, NPV Negative Predictive Value, 95% CI 95% confidence interval