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Table 7 AIV, NDV, IBV and IBDV detection in clinical specimens with use of different tests

From: New oligonucleotide microarray for rapid diagnosis of avian viral diseases

Result

Real-time RT-PCR

Microarray

Virus isolation in chicken embryos

Positive

117 (95.90%)

118 (96.72%)

118 (96.72%)

Falsepositive

0

0

0

Falsenegative

1 (0.82%)

1 (0.82%)

1 (0.82%)

Negative

4 (3.29%)

3 (2.46%)

3 (2.46%)

Total

122 (100%)

122 (100%)

122 (100%)

SN, at 95% CI

99.15% (97.30–100%)

99.16% (97.36–100%)

Standard method for comparison

SP, at 95% CI

100%

100%

PPV, at 95% CI

100%

100%

NPV, at 95% CI

80.00% (71.96–88.04%)

75.00% (66.29–83.71%)

  1. Abbreviations: SN Sensitivity, SP Specificity, PPV Positive Predictive Value, NPV Negative Predictive Value, 95% CI 95% confidence interval