Fig. 3
From: Hepatitis C virus NS4B protein induces epithelial-mesenchymal transition by upregulation of Snail
HCV NS4B promoted EMT due to upregulation of Snail. a HepG2 cells were transfected with pEGFPC1 plasmid (control group) and pEGFPC1-NS4B plasmid (NS4B group), 48 h later, the expression of Snail in control group and NS4B group. b HepG2 cells were transfected with pEGFPC1 plasmid (control group), 1, 3, 5 μg pEGFPC1-NS4B plasmid respectively, 48 h later, the expression of Snail in each group. c qRT-PCR analysis the mRNA expression of Snail in control group and NS4B group, The HepG2 cells were transfected with 3 μg pEGFPC1-NS4B plasmids in the qRT-PCR assay. d Western blotting analysis the expression of E-cadherin, N-cadherin, snail and HCV-NS4B in plasmids transfected cells, Snail siRNA for knockdown Snail. Co-transfected Snail siRNA and 3 μg pEGFPC1-NS4B plasmids into HepG2 cells, the expression level of Snail in HepG2 cells decreased after Snail siRNA transfection, and the phenomenons that downregulation of E-cadherin and upregulation of N-cadherin reversed while knockdown of snail. Con ~ for control group, n.s. ~ for no significant different, *P < 0.05, **P < 0.01