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Fig. 2 | Virology Journal

Fig. 2

From: Hepatitis C virus NS4B protein induces epithelial-mesenchymal transition by upregulation of Snail

Fig. 2

HCV NS4B induced hepatocellular EMT. a HepG2 cells were transfected with pEGFPC1 plasmid (control group) and pEGFPC1-NS4B plasmid (NS4B group), 48 h later, the expression of E-cadherin and N-cadherin in control group and NS4B group. b HepG2 cells were transfected with pEGFPC1 plasmid (control group), 1, 3, 5 μg pEGFPC1-NS4B plasmid respectively, 48 h later, the expression of E-cadherin and N-cadherin in each group. c qRT-PCR analysis the mRNA expression of E-cadherin and N-cadherin in control group and NS4B group. d Immunofluorescence analysis of E-cadherin and N-cadherin in control group and NS4B group. *P < 0.05, **P < 0.01

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