Fig. 4

HPV16-E2 protein promotes differentiation in HaCaT cells in a comparable way than epithelial differentiation inducers. a Western blot analysis showing the expression of cytokeratin 10 and 14 proteins in non-stimulated HaCaTwt cells, 5 days 5 mM CaCl₂ or 1 μM RA stimulated HaCaTwt cells, and HaCaT-HPV16-E2 cells. Numeric values below each lane are the quantitation results expressed as the ratio to non-stimulated HaCaTwt cells and normalized against β-actin. Flow - cytometric analysis. Left panels (b, d and f), HaCaTwt cells; right panels (c, e and g), HaCaT-HPV16-E2 cells. b and c, non-stimulated cells; d and e, CaCl₂ stimulated cells; f and g, RA stimulated cells. All images shown are representative of at least three independent experiments