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Table 2 The optimization of proteinase K concentration and the formalin fixed time

From: A novel ViewRNA in situ hybridization method for the detection of the dynamic distribution of Classical Swine Fever Virus RNA in PK15 cells

Proteinase K concentration

Formalin fixed 30 min

Formalin fixed 60 min

1:500

(-) Probe and (+) Probe

(-) Probe and (+) Probe

1:1000

(-) Probe and (+) Probe

(-) Probe and (+) Probe

1:2000

(-) Probe and (+) Probe

(-) Probe and (+) Probe

1:4000

(-) Probe and (+) Probe

(-) Probe and (+) Probe

  1. “(+)”Represents experimental group with probes,“(-)”Represents negative control without probes
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Virology Journal

ISSN: 1743-422X