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Table 2 The optimization of proteinase K concentration and the formalin fixed time

From: A novel ViewRNA in situ hybridization method for the detection of the dynamic distribution of Classical Swine Fever Virus RNA in PK15 cells

Proteinase K concentration Formalin fixed 30 min Formalin fixed 60 min
1:500 (-) Probe and (+) Probe (-) Probe and (+) Probe
1:1000 (-) Probe and (+) Probe (-) Probe and (+) Probe
1:2000 (-) Probe and (+) Probe (-) Probe and (+) Probe
1:4000 (-) Probe and (+) Probe (-) Probe and (+) Probe
  1. “(+)”Represents experimental group with probes,“(-)”Represents negative control without probes