A novel ViewRNA in situ hybridization method for the detection of the dynamic distribution of Classical Swine Fever Virus RNA in PK15 cells

Table 1 The samples background information of CSFV RNA ViewRNA ISH

Isolates and vaccines	Genotype	TCID₅₀	Infected cell	Source
SM	1.1	10^−3.67/200 μL	PK15	NCSFRL
C−Strain	1.1	10^−3.5/200 μL	ST	NCSFRL
SXDT2011	2.1	10^−3.3/200 μL	PK15	NCSFRL
HeBHH1/95	2.2	10^−4.5/200 μL	PK15	NCSFRL
HeNBY1/96	2.3		PK15	NCSFRL
PPV09/79			ST	CVCC
BVDV			MBDK	CVCC
PRV			PK15	CVCC
PCV-2			PK15	ZJU

SM shimen strain, F1114, C-Strain hog cholera lapinizedvaccine, PPV porcine parvovirus strain, BVDV OregonC24V strain, NADL strain, PRV porcine pseudorabies virus, AV1211, PCV-2 Porcinecirco virus II, PK15 Pig Kidney Passage Cell Lines, ST Swine Testicular Passage Cell Lines, MBDK Bovine Kidney Cell; NCSFRL National Classical Swine Fever Reference Lab, CVCC China Veterinary Culture Collection Center; ZJU Zhejiang University