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Fig. 5 | Virology Journal

Fig. 5

From: Study of SV40 large T antigen nucleotide specificity for DNA unwinding

Fig. 5

Characterization of ATP binding by LT using isothermal titration calorimetry (ITC). a Raw titration curves of WT ± Mg++ and different nucleotide pocket mutants in the presence of Mg++. Thirteen titrations were measured (μcal/second) for each curve over seventy minutes. b Graphical representation of the trends in total heat ΔQ changes over each injection. c Quantification of ΔQ over each injection. The negative values indicate an exothermic release of heat. The syringe (ATP) and cell (LT protein) concentrations in each experiment were 400uM and 25uM, respectively and the storage buffer contained 25 mM Tris pH 8.0, 250 mM NaCl, 0.5 mM TCEP, and 1 mM MgCl2 unless otherwise noted

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