Fig. 3
From: Study of SV40 large T antigen nucleotide specificity for DNA unwinding
Helicase assay results of the combined double-mutants of hydrophobic residues around the nucleotide pocket. a The assay results of the unwinding activities of LT mutants’ cW393A/cL397A and cL553A/cL557A. Conditions for the helicase assays are the same as described in Fig. 2. b The position of each set of residues in the nucleotide binding pocket with cW393A/cL397A located on α-helix 7 (purple) and cL553A/cL557A on α-helix 13 (orange) of the LT hexameric helicase structure. P-loop is colored in yellow as a point of reference