Table 1 Primer sequences, annealing temperatures and amplicon lengths, obtained in nested PCR assays for SNPs in the TLR genes
From: Toll-like receptors genes polymorphisms and the occurrence of HCMV infection among pregnant women
Gene | GenBank Accession No.a | SNPb name | Primer sequences (5’-3’) | Annealing temperature [oC] | Amplicon length (bps) c | |
|---|---|---|---|---|---|---|
TLR2 | NC_000004.12 | 2258 G > A (rs5743708) | External | For: CGGAATGTCACAGGACAGC Rev: GGACTTTATCGCAGCTCTCAG | 52 | 605 |
Internal | For: GCCTACTGGGTGGAGAACCT Rev: GGCCACTCCAGGTAGGTCTT | 59 | 340 | |||
TLR4 | NG_011475 | 896 A > G | External | For: AAAACTTGTATTCAAGGTCTGGC | 52 | 355 |
(rs4986790) | Rev: TGTTGGAAGTGAAAGTAAGCCT | |||||
Internal | For: AGCATACTTAGACTACTACCTCCATG | 61 | 188 | |||
Rev: AGAAGATTTGAGTTTCAATGTGGG | ||||||
1196 C > T | External | For: AGTTGATCTACCAAGCCTTGAGT | 52 | 510 | ||
(rs4986791) | Rev: GGAAACGTATCCAATGAAAAGA | |||||
Internal | For: GGTTGCTGTTCTCAAAGTGATTTTGGGAGAA | 59 | 407 | |||
Rev: ACCTGAAGACTGGAGAGTGAGTTAAATGCT | ||||||
TLR9 | EU170539 | 2848 G > A | External | For: GTCAATGGCTCCCAGTTCC | 52 | 292 |
(rs352140) | Rev: CATTGCCGCTGAAGTCCA | |||||
Internal | For: AAGCTGGACCTCTACCACGA | 59 | 177 | |||
Rev: TTGGCTGTGGATGTTGTT | ||||||