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Table 2 Mutations in vTK gene, susceptibility to ACV, and virulence of HSV-1 clones

From: Association between sensitivity of viral thymidine kinase-associated acyclovir-resistant herpes simplex virus type 1 and virulence

HSV-1 clone Mutations in vTK Accession number IC50 of ACV (that in the previous study [18]), (μg/ml) LD50 (PFU/head)
  Nucleotidea Amino acida    Intracerebral inoculation Corneal inoculation
TAS No No AB047358 1.2 × 100 (6.0 × 10−1) 1.3 × 10−1 1.1 × 103
CL1 G added within 7G (430–436) AB047359 1.1 × 102 (>1.0 × 102) 1.3 × 103 >1.0 × 106 c
CL18 C194A Thr65Asnb AB047372 5.6 × 100 (4.8 × 100) 7.5 × 100 4.9 × 104
CL19 C250T Pro84Ser AB047373 2.1 × 101 (1.6 × 101) 1.0 × 100 1.9 × 104
CL22 C734T Thr245Met AB047376 7.8 × 101 (8.0 × 101) 1.0 × 102 > 1.0 × 106
CL24 G1007A Cys336Tyr AB047378 5.6 × 101 (7.2 × 101) 3.2 × 101 > 1.0 × 106
  1. a‘C194A’ represents the nucleotide substitution of cytosine (C) for adenine (A) at position 194
  2. b‘Thr65Asn’ represents the amino acid substitution of threonine (Thr) for asparagine (Asn) at position 55
  3. cAll mice infected with CL1, 22, 24 survived even at an input dose of 106 PFU