Association between sensitivity of viral thymidine kinase-associated acyclovir-resistant herpes simplex virus type 1 and virulence

Table 2 Mutations in vTK gene, susceptibility to ACV, and virulence of HSV-1 clones

HSV-1 clone	Mutations in vTK		Accession number	IC₅₀ of ACV (that in the previous study [18]), (μg/ml)	LD₅₀ (PFU/head)
	Nucleotide^a	Amino acid^a			Intracerebral inoculation	Corneal inoculation
TAS	No	No	AB047358	1.2 × 10⁰ (6.0 × 10⁻¹)	1.3 × 10⁻¹	1.1 × 10³
CL1	G added within 7G (430–436)		AB047359	1.1 × 10² (>1.0 × 10²)	1.3 × 10³	>1.0 × 10^{6 c}
CL18	C194A	Thr65Asn^b	AB047372	5.6 × 10⁰ (4.8 × 10⁰)	7.5 × 10⁰	4.9 × 10⁴
CL19	C250T	Pro84Ser	AB047373	2.1 × 10¹ (1.6 × 10¹)	1.0 × 10⁰	1.9 × 10⁴
CL22	C734T	Thr245Met	AB047376	7.8 × 10¹ (8.0 × 10¹)	1.0 × 10²	> 1.0 × 10⁶
CL24	G1007A	Cys336Tyr	AB047378	5.6 × 10¹ (7.2 × 10¹)	3.2 × 10¹	> 1.0 × 10⁶

^a‘C194A’ represents the nucleotide substitution of cytosine (C) for adenine (A) at position 194
^b‘Thr65Asn’ represents the amino acid substitution of threonine (Thr) for asparagine (Asn) at position 55
^cAll mice infected with CL1, 22, 24 survived even at an input dose of 10⁶ PFU

ISSN: 1743-422X