Fig. 4

Inhibitory activities of N30 against CVB3, RSV and coronavirus. a The effect of N30 on CVB3 VP1 protein and mRNA expression in Vero cells. N30 was added to Vero cells after the cells were infected with Coxsackie B virus (0.005 MOI) for 2 hours, CVB3 VP1 mRNA and protein were extracted at 24 h post-infection. b The inhibitory effect of N30 on expression of RSV M2 mRNA and protein in Hep2 cells. N30 was added to Hep2 cells simultaneously with RSV (0.025 MOI) infection. RSV M2 mRNA and protein were extracted at 24 h post-infection. c The effect of N30 on coronavirus. N30 was added to MRC-5 cells after infection with coronavirus (0.005 MOI) for 2 hours. Total cellular RNA and proteins was collected 24 h after infection. All the experiments were performed with 5 μM RBV or DMSO as control. The experiments were performed in triplicate, and the data represents mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 versus Con