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Fig. 1 | Virology Journal

Fig. 1

From: A novel benzo-heterocyclic amine derivative N30 inhibits influenza virus replication by depression of Inosine-5’-Monophospate Dehydrogenase activity

Fig. 1

Effects of N30 on IAV M2 protein and RNA expression in MDCK. a Chemical structure of N30. b The effect of N30 on viabilities of MRC-5, Vero, MDCK and Hep2 cells, which were measured by CCK. c The effect of N30 with different concentrations on IAV M2 protein and RNA expression. d The effect of N30 on IAV M2 protein and RNA expression compared with reference drugs. MDCK cells were infected with IAV A/Fort Monmouth/1/1947(H1N1) (0.01 multiplicity of infection [MOI, plaque-forming units (PFU)/cell]) for 2 hours, and treated with different concentrations of N30 or reference drugs for 24 h. Total mRNA and protein were extracted at 24 h post-infection, and analyzed by qRT-PCR and Western blotting respectively. Mock: normal cells without treatment; Con: infected cells treated with equal amounts of DMSO as N30. The experiments were performed in triplicate, and the data represents mean ± SD. ***P < 0.001 versus Con (ANOVA)

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Virology Journal

ISSN: 1743-422X