Table 1 Primers used for amplification of the p10.8- and σNS-encoding genes of MDRV
From: Muscovy duck reovirus σNS protein triggers autophagy enhancing virus replication
Gene | Primers | Primer sequence (5′-3′) | Expected size |
|---|---|---|---|
p10.8 | P1F | CGAATTCCATGGCTGATGCTTTTGAAGT (EcoR I) | 288 bp |
P2R | TTGCGGCCGCCTAGTTAGATCCCGAGAG (Not I) | ||
P3Fa | CGTGTCCTGTCGGTCTTAGC | 125 bp | |
P4Ra | TGAAGGTGGTATTCGTTCCAG | ||
σNS | P5F | CCACGCGTACCCATGGACAACACCGT (Mlu I) | 1104 bp |
P6R | TCCTGTCGACCTACGCCATCCTAGCTG (Sal I) | ||
GAPDH | P7Fa | TGCTAAGCGTGTCATCATCT | 187 bp |
P8Ra | AGTGGTCATAAGACCCTCCA |