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Fig. 6 | Virology Journal

Fig. 6

From: Muscovy duck reovirus σNS protein triggers autophagy enhancing virus replication

Fig. 6

The MDRV σNS nonstructural protein is involved in MDRV-induced autophagy. DF-1 cells were cultured in 6-well plates and transfected with plasmids containing the respective p10.8 and σNS genes of MDRV. Mock-transfected and vector-transfected cells were used as controls. Samples were collected at indicated time points and analyzed with Western-blot assay using specific antibodies against LC3 I/II and β-actin. The MDRV p10.8 (left panel) and σNS (right panel) proteins were detected with rabbit polyclonal antibodies. The activation and inactivation folds in each treatment indicated below each lane were normalized against those at 6 h. The proteins levels were normalized to those for β-actin. The levels of indicated proteins at 6 h were considered 1 fold. Similar results were obtained in three independent experiments

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