Fig. 4
From: Muscovy duck reovirus σNS protein triggers autophagy enhancing virus replication
Autophagy regulators rapamycin and chloroquine affect virus yield. DF-1 cells cultured in 6-well plate were pretreated with rapamycin (100 nM) and chloroquine (50 μM) or mock-treated for 1 h, followed by MDRV infection with at an MOI of 1. Relative mRNA level of the p10.8 gene in MDRV-infected DF-1 cells was determined with real-time RT-PCR (*p < 0.05; **p < 0.01), and the mRNA levels of the p10.8 gene of MDRV was normalized relative to that of the housekeeping GAPDH gene. All the data shown represent the mean ± SD calculated from three independent experiments