Fig. 5
Alignment of amino acid residues defining the neutralization domains in VP8* subunit (8-1, 8-2, 8-3 and 8-4) of VP4 of rotavirus strains analyzed. Deduced amino acid sequences of approximately 330 residues, corresponding to the VP8* and partially VP5* subunit of VP4, were analyzed from rotavirus strains circulating in Caracas (2007/2008) and Valencia (2003) (in bold) and compared with reference strains. For each strain, host species, country of origin, year of isolation and genotypes G-P are shown. Numbering is based on Rotarix®-A41CA419A vaccine strain sequence in use in Venezuela during 2007-2008. a Neutralization domains from P[8] genotype strains analyzed in this study and global reference strains. Identical amino acids with Rotarix® vaccine strain in each isolate are identified by dots. Amino acid differences exhibited only by the RV P[8] strains from Caracas circulating in 2008, in comparison to any other P[8] strain analyzed, are in box. b Neutralization domains from P[4] genotype strains analyzed in this study and global reference strains. Identical amino acids with the prototype G2 strain DS-1 in each isolate are identified by dots. Amino acid changes that have been shown to escape neutralization with monoclonal antibodies [40] are indicated with a filled triangle (▲)