Fig. 4
Alignment of amino acid residues defining the neutralization domains in VP7 protein (7-1a, 7-1-b and 7-2) of rotavirus strains analyzed. Deduced amino acid sequences of approximately 270 residues were obtained from rotavirus strains circulating in Caracas (2007/2008) and Valencia (2003) (in bold) and compared with reference strains. For each strain, host species, country of origin, year of isolation and genotypes G-P are shown. Numbering is based on Rotarix®-A41CA419A vaccine strain sequence used in Venezuela during the years 2007-2008. The sites not included in the analysis were indicated with a hyphen (-). a Neutralization domains from G1 genotype strains analyzed in this study and global reference strains. Identical amino acids with Rotarix® strain in each isolate are identified by dots. Amino acid residue differences among the G1 strains circulating in Venezuela during 2007/2008 season and the pre-vaccine 2003 G1 from Valencia, or Rotarix® vaccine strain, are in boxes. b Neutralization domains from G2 genotype strains analyzed in this study and global reference strains. Identical amino acids with the prototype G2 strain DS-1 isolated in 1976 in each isolate are identified by dots. Amino acid residue differences among the G2 strain from Caracas 2007 and the pre-vaccine from Valencia 2003, are in boxes. Amino acid changes that have been shown to escape neutralization with monoclonal antibodies [69] are indicated with a filled triangle (▲)