Fig. 1

Representative RNA patterns obtained by polyacrylamide gel electrophoresis (PAGE) of rotavirus strains isolated from children with diarrhea from Caracas. Rotavirus RNA segments extracted from fecal samples were separated in a 7% polyacrylamide gel at room temperature for 3 h at 90 V and visualized by staining with silver nitrate as described previously [33]. All the isolates exhibited a profile of group A rotavirus (4-2-3-2). The standard molecular weight (1 Kb Plus DNA Ladder, Invitrogen^TM, CA, USA) (lane 1), one RNA short (slower-moving gene segment 11) (lane 2) and three RNA long (faster-moving gene segment 11) patterns (lane 3–5) of RV genomes are shown