From: RNA extraction method is crucial for human papillomavirus E6/E7 oncogenes detection
Analysis of each extraction method separately | ||
Method | Cohen’s kappa coefficient κ | |
Pathology | Nuclisens manual extraction kit (bioMérieux) | 0.26 |
High Pure Viral RNA Kit (Roche) | 0.32 | |
Lesion progression | Nuclisens manual extraction kit (bioMérieux) | 0.26 |
High Pure Viral RNA Kit (Roche) | 0.41 | |
Comparison between biomerieux and Roche extraction methods | ||
E6/E7 mRNA detection rate | 0.26 |