RNA extraction method is crucial for human papillomavirus E6/E7 oncogenes detection

Table 2 The relative observed agreement concerning pathology and lesion progression in two RNA extraction methods

Analysis of each extraction method separately
	Method		The relative observed agreement Pr(a)
Pathology	Nuclisens manual extraction kit (bioMérieux)	No lesion	0.5
		ASCUS + LSIL	0.72
		HSIL	1
	High Pure Viral RNA Kit (Roche)	No lesion	0.89
		ASCUS + LSIL	0.33
		HSIL	0.75
Lesion progression	Nuclisens manual extraction kit (bioMérieux)	Same lesion	0.52
	Nuclisens manual extraction kit (bioMérieux)	Worsened lesion	0.74
	High Pure Viral RNA Kit (Roche)	Same lesion	0.95
	High Pure Viral RNA Kit (Roche)	Worsened lesion	0.40
Comparison between biomerieux and Roche extraction methods
E6/E7 mRNA detection rate			0.58
Pathology		No lesion	0.61
		ASCUS + LSIL	0.50
		HSIL	0.75
Lesion progression		Same lesion	0.57
Lesion progression		Worsened lesion	0.60

Pathology was categorized into three groups: 1) Normal (no lesion), 2) Atypical squamous cells of undetermined significance (ASCUS) or low-grade squamous intraepithelial lesion (LSIL) and, 3) High-grade squamous intraepithelial lesion (HSIL). Lesion progression was divided into two groups: 1) women whose cytological results had not changed in the last two years and, 2) women whose cytological results showed a worsened process

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