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Table 1 Primer sequences used in this studyNote

From: C-terminal region of apoptin affects chicken anemia virus replication and virulence

Name Sequences The position with point mutation
88U 5’-CTCCCTCGAAGAAGTGATCCTGCGACCCCTC-3’ 747
88 L 5’-GAGGGGTCGCAGGATCACTTCTTCGAGGGAG-3’ 747
80U 5’-CTTGAGGACCGATTAACCCAAGCCTCCCTCG-3’ 723
80 L 5’- CGAGGGAGGCTTGGGTTAATCGGTCCTCAAG-3’ 723
Utu 5’-CATACCGGTCGGCAGTAG-3’ -
Ltu 5’-CGATACCGCTGTCTCCTC-3’ -
IU 5’-GCTGGT A CCGCTCGGCACGGAGAC-3’ 1902
IL 5’-AGGGG T ACCAGCGTGTGCCATCTC-3’ 1902
  1. NoteMutated nucleotides were in bold in the primer sequences, and the restriction enzyme sites were underlined