From: Hepatitis C Virus in mainland China with an emphasis on genotype and subtype distribution
Region | Province | Genotyping method | Amplified fragment |
---|---|---|---|
Central China1* | Hunan, Hubei, Henan | Nested-PCR and sequence analysis, PCR-RDB, GT-specific primers PCR method, gene chip assay | Core and E1, NS5B and/or Core-E1, NS5B, 5’UTR, core |
East China2* | Shandong, Jiangsu, Anhui, Jiangxi, Fujian, Shanghai, Zhejiang | Nested-PCR and sequence analysis, RFLP, GT-specific primers PCR method, gene chip assay, Genotyping detection kit (DNA sequence assay), PCR-RDB, Micro plate nucleic acid hybridization-ELISA technique, LiPA | 5’-UTR, core, NS5B and/or core-E1, 5’-UTR and/or NS5B, NS5B and C/E2, core and NS5B and 5’-UTR, E2, NS5B and E2 |
South China3* | Guangdong, Guangxi, Hainan | Nested-PCR and sequence analysis, GT-specific primers PCR method, gene chip assay, PCR – RDB, LiPA | NS5B and E1, core and NS5B, E1, NS5B, 5’-UTR and NS5B |
North China4* | Beijing, Shanxi, Tianjin, Hebei, IM | RFLP, Nested-PCR and sequence analysis, GT-specific primers PCR method, gene chip assay, PCR fluorescent probe method | Core, C/E1, 5’-UTR |
Southwest5* | Sichuan, Yunnan, Chongqing, Guizhou | gene chip assay, RFLP, Nested-PCR and sequence analysis, PCR-RDB, Type specific probe hybridization method | 5’-UTR, 5’UTR-core and E1-E2 and NS5B, 5’NCR-C and NS5B, core/E1 and NS5B, E1/E2 and NS5B, 5’-UTR and Core, core and E1, core, C/E1, NS5B |
Northwest6* | Gansu, Sha anxi, Xinjiang | Nested-PCR and sequence analysis, RFLP, PCR-RDB,GT-specific primers PCR method,LiPA, restriction endonuclease cleaving method | E1 and NS5B, 5’NCR, NS5B |
Northeast7* | Liaoning, Heilongjiang, Jilin | RFLP, Nested-PCR and sequence analysis, PCR – RDB, type-specific primers PCR method, gene chip assay | NS5, Core, 5’UTR, C and E1 and NS5 |