Fig. 3

VP16 inhibits the early induction of viperin from MAVS-Pex. a HEK293 cells were pretreated with 50 ng/ml BFA and transfected with empty vector, MAVS-Pex-HA and individual HSV-1 protein expression plasmid in the presence of BFA. At 18 h post transfection, cells were harvested and subjected to qRT-PCR analysis. b HEK293 cells were pretreated with 50 ng/ml BFA and transfected with MAVS-Pex-HA and VP16-Flag plasmids as indicated in the presence of BFA. At 18 h post transfection, cells were harvested and subjected to WB analysis with anti-viperin antibody. The data represent results from one of the triplicate experiments