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Fig. 2 | Virology Journal

Fig. 2

From: Herpes simplex virus 1 infection dampens the immediate early antiviral innate immunity signaling from peroxisomes by tegument protein VP16

Fig. 2

HSV-1 infection at a high MOI dampens the immediate early induction of viperin triggered by MAVS-Pex. a MEF cells were transfected with MAVS-Pex-YFP, along with PTS1-DsRed plasmid for 36 h. The images were obtained by fluorescence microscopy using a 20× objective. b HEK293 cells were pretreated with or without 50 ng/ml BFA and transfected with empty vector or MAVS-Pex-HA plasmid for 18 h. Then, the supernatants were collected and added to Viperin-Luc and pRL-TK transfected cells for DLR analysis. (c and d) HEK293 cells were pretreated with 50 ng/ml BFA and infected with or without WT HSV-1 at an MOI of 5. At 2 h post infection, the cells were transfected with the indicated plasmids in the presence of BFA. Eighteen hours post transfection, cells were harvested and subjected to RT-PCR (c) and WB (d) analysis. The data represent results from one of the triplicate experiments

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