Skip to main content
Fig. 3 | Virology Journal

Fig. 3

From: The DNA damage response promotes polyomavirus JC infection by nucleus to cytoplasm NF- kappaB activation

Fig. 3

Role of NEMO cytoplasm to nucleus translocation in JCV infection: Analysis of the effect of JCV infection on the subcellular distribution of NEMO by cell fractionation. SVGA cells were transfected with expression plasmid for FLAG-NEMO and infected with wild-type Mad-1 JCV 24 h later. Cells were harvested at 1, 3, 4 and 5 d after infection and fractionated into cytoplasmic and nuclear fractions. Western blot for α-FLAG was used to monitor the subcellular distribution of NEMO. Western blots for α-tubulin and lamin A/C were used to monitor cell fraction purity and for VP1 to monitor infection

Back to article page

Virology Journal

ISSN: 1743-422X