Fig. 1
Location of changes in the transmembrane region of HIV-1JR-FL Envs. a The projection of the helical coiled coil in the HIV-1 Env transmembrane region, based on the NMR structure of a peptide embedded in bicelles [29], is shown. Six hydrophobic residues (I688, L692, L695, V698, L702 and V705) highlighted in red are predicted to be located on the lipid-interacting surface of the membrane-spanning coiled coil. b In the TMmod1-17 constructs, the six hydrophobic residues on the putative lipid-interacting surface in a were changed to combinations of alanine, glutamine or charged amino acid residues. c The TMmod10v2 construct is a TMmod10 variant with three additional changes (M687D, L697A and F699A) introduced to modify all of the predicted external positions b, c, e, f, g on the helical coiled coil. d The TMmod10v3 construct is identical to TMmod10v2 except that the residues in the e and g positions of the coiled coil (L692, L697 and F699) are wild-type in sequence. e The TMmod18 glycoprotein control contains four changes in the transmembrane region, three of which (I686E, V693K and T700E) are predicted to be located in the interior of the coiled coil (at the a position of the heptad repeat sequence)