Partial HIV C2V3 envelope sequence analysis reveals association of coreceptor tropism, envelope glycosylation and viral genotypic variability among Kenyan patients on HAART

Table 3 The distribution and clustering of specific amino acid PNG sites according to viral tropism and source material

Number of isolates; % possessing the shown amino acid PNG at specified site
	N262	N277	N296	N302	N337	N345	N366	N399	N408	Total
NA source
DNA	33; 82.5	40; 100	28; 70	39;97.5	25;62.5	30; 75	32; 80	35;87.5	33; 82.5	40; 100
RNA	11; 73.3	14;93.3	10;66.7	15; 100	8; 53.3	10;66.7	9; 60	8; 53.3	7; 46.7	15; 100
Tropism
CCR5	31; 73.8	41;97.6	29;69.0	41;97.6	25;59.5	30;71.4	33;78.6	31;73.8	29; 69.0	42; 100
CXCR4	13; 100	13;100	9; 69.2	13;100	8; 61.5	10;76.9	8; 61.5	12;92.3	11; 84.6	13; 100
Subtype
A1	28; 77.8	36; 100	24; 66.7	36; 100	18; 50	27; 0.75	30; 83.3	25; 69.4	26; 72.2	36; 100
A1r^a	4; 80	4; 80	5; 100	5; 100	4; 80	3; 60	2; 40	4; 80	3; 60	5; 100
A2	3;75	4;100	4;100	4;100	3;75	2;50	3;75	4;100	2;50	4;100
C	4;80	5;100	1;20	4;80	4;80	3;60	5;100	5;100	4;80	5;100
D	5;100	5;100	4;80	5;100	4;80	5;100	1;20	5;100	5;100	5;100
Overall	44; 80	54;98.2	38;69.1	54;98.2	33; 60	40;72.7	41;74.5	43;78.2	40; 72.7	55;100

Key: NA Nucleic Acid. Tropism analysis is based on false positive rate of 10%. ^aA1r; A1A2 (n = 1), A1D (n = 3), and A1A2D (n = 1). Mean PNG differences are significant between subtypes at specific A.A positions N277 (p = 0.034), N296 (p = 0.036), N302 (p = 0.034) and N366 (p = 0.004) in Anova tests

ISSN: 1743-422X