Fig. 4From: Identification of host cellular proteins that interact with the M protein of a highly pathogenic porcine reproductive and respiratory syndrome virus vaccine strainColocalization of M protein with NF45 and PCNA. 293Â T cells were cotransfected with HA-M and Flag-NF45/PCNA. Cells were fixed at 48Â h and subjected to indirect immunofluorescence to detect HA-M (green) and Flag-NF45/PCNA (red) with mouse anti-HA and rabbit anti-Flag antibodies. The position of the nucleus is indicated by DAPI (blue) staining in the merged imageBack to article page