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Fig. 3 | Virology Journal

Fig. 3

From: Identification of host cellular proteins that interact with the M protein of a highly pathogenic porcine reproductive and respiratory syndrome virus vaccine strain

Fig. 3

Confirmation of the interaction of PRRSV M protein with NF45 and PCNA by CO-IP. The interaction of M protein and exogenous NF45 and PCNA. 293 T cells were co-transfected with 5 μg of the indicated plasmids in 60-mm dishes. Cell lysates were prepared at 36–48 h after transfection and the proteins were immunoprecipitated with anti-Flag mAb. Proteins in cell lysates (input) and immunoprecipitated samples were detected with the antibodies against Flag, HA, and M by Western blot. The asterisk (*) indicates IgG (Flag mAb) heavy chains. a NF45 and PCNA were immunoprecipitated by M protein. b M protein was immunoprecipitated by NF45 and PCNA

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