Skip to main content
Fig. 1 | Virology Journal

Fig. 1

From: Identification of host cellular proteins that interact with the M protein of a highly pathogenic porcine reproductive and respiratory syndrome virus vaccine strain

Fig. 1

Identification of the cellular proteins that interact with PRRSV M protein by co-immunoprecipitation (Co-IP). a Cell lysates from HP-PRRSV strain HuN4-F112-infected MARC-145 cells at different time points were subjected to Western blot with anti-M protein mAb 3 F7 and anti-GAPDH mAb. b Cell lysates from HuN4-F112/HuN4-F5- or mock-infected MARC-145 cells were immunoprecipitated with anti-M protein mAb 3 F7. The three lanes (lane 1, lane 2, and lane 3) stand for HuN4-F5 infected group, HuN4-F112 infected group, and control group, respectively. The immunoprecipitated proteins were separated by 12% SDS-PAGE and visualized by Coomassie brilliant blue staining. The asterisks (*) show the differential protein bands between HuN4-F112- or mock-infected MARC-145 cells. c The experimental procedure was as the same as above, but the cell lysates were subjected to Western blot with anti-M protein mAb 3 F7

Back to article page
\