Fig. 3

Evaluation of influenza-specific IgA response. Mice were vaccinated intranasally or subcutaneously three times with three week-interval using split influenza antigen (A/California/07/2009(H1N1)pdm) with or without the adjuvant Endocine™. Influenza-specific IgA (a) in nasal wash and (b) lung homogenate. c Total IgA in lung homogenate. Data shown represent geometric mean titers (GMT). For data which included values of 0 (1 mouse in the non-adj s.c. 20 month group, and 2 mice in the non-adj s.c. 2 month group), a value of 0.01 was added to calculate GMT. Significant differences to the adjuvanted vaccine group is indicated by *p < 0.05, **p < 0.01, ***p < 0.001