Fig. 3

Predicted location of amino acids in the HA and NA proteins of A/turkey/Egypt/AR1507/2016(H5N1). Shown are mutations in HA (a) and NA (b) in TK16 compared to the vaccine strain Dk2006. (a) Most of mutations in the HA occurred in the head domain (transparent grey circle): mutations in the immunogenic epitopes (red), receptor binding sites (blue), de-glycosylated sites (green), in the proteolytic cleavage site (yellow) and those with unknown function (cyan). (b) in the NA, mutations occurred in the stalk (n = 11; while residues 20, 29, 34, 44 and 46 are not shown in the 3D structure) and head domain (n = 17), the sialidase functional and framework sites (magenta), mutations in the immunogenic epitopes (red), glycosylated sites (green), and those with unknown function (cyan) are shown. All mutations are imposed on the HA and NA of the parent A/chicken/Egypt/NLQP-06207/2006 virus