Fig. 6

NPbd3 maintains nucleic acid binding, oligmerization, and vRNP formation. a WT NP and NPbd3 were immuno purified using anti-FLAG antibody. Purified WT NP and NPbd3 were incubated with biotin labeled single stranded DNA and separated on an 8% PAGE TBE non denaturing gel before transfer to nitrocellulose. Biotin ssDNA was detected by interaction with Streptavidin-HRP and ECL reagents. No NP samples expressed no NP protein and serve as negative control. b Blue Native Gel Electrophoresis was utilized to migrate protein extracts of cells expressing NP and NPbd3 followed by western blot with anti-FLAG antibody to demonstrate NP oligomer formation. Asterisk represents Native Mark Unstained Protein standard at 480 kDa. c Blue Native Gel Electrophoresis was utilized to migrate protein extracts of cells expressing reconstituted vRNPs comprised of either NP or NPbd3 followed by western blot with anti-FLAG antibody to demonstrate vRNP formation. Asterisk represents Native Mark Unstained Protein standard at 1048 kDa