Fig. 8

Perforin is produced by CD8⁺ T lymphocytes in the spleen of C57BL/6 mice after infection. Groups of C57BL/6 (WT) and TLR2/9^−/− (KO) mice (6 animals/group) were infected with 10⁶ p.f.u. HSV-1 via the intranasal route and, on the 5^th day post infection, mice were euthanized, and TG and spleen cell suspensions prepared for flow cytometry analysis. The frequency of each cell within the live leukocyte population was determined. a Frequencies of perforin-producing CD4⁺ T cells (CD45⁺CD3⁺CD4⁺); b CD8⁺ T cells (CD45⁺CD3⁺CD8⁺); c NK T cells (CD45⁺CD3⁺NK1.1⁺) and d NK cells (CD45⁺NK1.1⁺). A minimum of 100,000 events was acquired for analysis. White bars: mock-infected mice; black bars: infected mice. Bars show the means ± SEM. * p < 0.05; ** p < 0.01 and *** p < 0.001