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Fig. 1 | Virology Journal

Fig. 1

From: Dendritic cells, macrophages, NK and CD8+ T lymphocytes play pivotal roles in controlling HSV-1 in the trigeminal ganglia by producing IL1-beta, iNOS and granzyme B

Fig. 1

DCs and monocyte/macrophage are higher in C57BL/6 infected than in mock-infected mice, differently of TLR2/9−/−. The profile of immune cells present in the TG and spleen of C57BL/6 (WT) and TLR2/9−/− (KO) mice was assessed by cytometry analysis. Groups of six mice were infected with 106 p.f.u. of HSV-1 via the intranasal route and, on the 5th day post infection, mice were euthanized, and TG and spleen cell suspensions were prepared. a Frequencies of live leukocytes (CD45+) were analyzed within the population of TG and spleen cells and frequencies of myeloid cells (CD45+CD3CD11b+) were analyzed within the population of live leukocytes in the TG and spleen; b CD4 (CD45+CD3+CD4+), CD8 (CD45+CD3+CD8+), NK T (CD45+CD3+NK1.1+), NK (CD45+NK1.1+), dendritic (DC, CD45+MHC class IIhigh+CD11c+) and monocyte/macrophage (Mo/Mϕ, CD45+F4/80+) cells were analyzed within the population of live leukocytes in the TG and spleen. A minimum of 100,000 events was acquired for analysis. Bars show the means ± SEM. * p < 0.05; ** p < 0.01 and *** p < 0.001. White bars: mock-infected mice; black bars: infected mice

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