Fig. 4

Kinetics of viral progeny in several tissues following CV-B4 E2 inoculation at either gestational day 10 (a) or 17 (b). Uteri from two pregnant dams inoculated at day 10G, together with amniotic sacs, amniotic fluids (pool), umbilical cords and placentas of three offspring of each dam, were sampled at day 17G. Pancreases and hearts from six offspring (each three born to one different dam) were equally sampled at each of days 17G, and 0 and 5 post-partum when dams were inoculated at day 10G, and at days 0 and 5 post-partum when dams were inoculated at day 17G. Samples were subjected to viral progeny titration by the Reed and Muench’s method as described in the Methods section. Results are plotted as mean TCID ₅₀ /mg (except for the amniotic fluid where they are expressed as mean TCID ₅₀ /ml) ± SD, n = 6. No trace of viral progeny could be evidenced in any tissue from all the negative control animals