Fig. 3

Kinetics of anti-CV-B4 neutralizing antibodies after virus inoculation of pregnant dams. In order to evaluate the presence and the amount of anti-CV-B4 neutralizing antibodies, seroneutralization was performed at different time-points on (a): sera from dams inoculated with CV-B4 E2 at either day 10G or 17G, (b): supernatants of homogenized internal tissues from offspring born to dams inoculated with CV-B4 E2 at either day 10G or 17G and (c): sera from offspring (at least 21 days old) born to dams inoculated with CV-B4 E2 at day 17G. Results are plotted as mean neutralizing titers ± SD, n = 2 for dams and n = 6 for offspring. The proportion of seropositive animals is indicated on each bar. Neutralizing antibodies were not detected in any sample from all negative control mice